Anti-BDNF Antibody (56572) from QED Bioscience Inc.

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (56572). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.
Western Blot

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
Western Blot

Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.

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QED Bioscience Inc. for
Anti-BDNF Antibody (56572)

The Anti-BDNF Antibody (56572) from QED Bioscience Inc. is a Rabbit Polyclonal antibody to BDNF, and Brain Derived Neurotrophic Factor. This antibody recognizes Homo Sapiens, Human, and Mouse antigen. The Anti-BDNF Antibody (56572) has been validated for the following applications: Immunocytochemistry, Immunofluorescence, and Western Blot.

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Western Blot

Western Blot

Anti-BDNF Antibody (56572) from QED Bioscience Inc.

QED Bioscience Inc.

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Anti-BDNF Antibody (56572) from QED Bioscience Inc.

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